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BLC Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT5208
产品名称
BLC Rabbit Polyclonal Antibody
别名
CXCL13; BCA1; BLC; SCYB13; C-X-C motif chemokine 13; Angie; B cell-attracting chemokine 1; BCA-1; B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13
类别
常规抗体
基因名称
CXCL13
蛋白名称
C-X-C motif chemokine 13
推荐应用
WB
反应种属
Human,Rat,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
10563
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10563
Human Swissprot No.
O43927
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43927/entry
Mouse Swissprot No.
O55038
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O55038
免疫原
The antiserum was produced against synthesized peptide derived from the Internal region of human CXCL13. AA range:41-90
特异性
BLC Polyclonal Antibody detects endogenous levels of BLC protein.
稀释度
WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000.. IF 1:50-200
预测分子量
15kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
C-X-C motif chemokine ligand 13(CXCL13) Homo sapiens B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq, Oct 2014],
组织表达
Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.
细胞定位
Secreted.
信号通路
Cytokine-cytokine receptor interaction;Chemokine;
功能
function:Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.,online information:CXCL13 entry,similarity:Belongs to the intercrine alpha (chemokine CxC) family.,tissue specificity:Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from mouse spleen cells, using CXCL13 Antibody.

Western Blot analysis of mouse spleen cells using BLC Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-muscle, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human Liver. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Liver. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Liver. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

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