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IL-1β Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT5201
产品名称
IL-1β Rabbit Polyclonal Antibody
别名
IL1B; IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin
类别
常规抗体
基因名称
IL1B
蛋白名称
Interleukin-1 beta
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
3553
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3553
Human Swissprot No.
P01584
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P01584/entry
Mouse Gene ID
16176
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16176
Mouse Swissprot No.
P10749
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P10749
Rat Swissprot No.
Q63264
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q63264
免疫原
The antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range:181-230
特异性
IL-1β Polyclonal Antibody detects endogenous levels of IL-1β protein.
稀释度
WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.
预测分子量
17kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008],
组织表达
Expressed in activated monocytes/macrophages (at protein level).
细胞定位
Cytoplasm, cytosol . Secreted . Lysosome . Secreted, extracellular exosome . The precursor is cytosolic (PubMed:15192144). In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted (PubMed:24201029, PubMed:33377178, PubMed:33883744). Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744). In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation (PubMed:33883744). The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10 (PubMed:32272059). .
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;Cytokine-cytokine receptor interaction;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Toll_Like;NOD-like receptor;Cytosolic DNA-sensing pathway;Hematopoietic cell lineage;Type I diabetes mellitus;Alzheimer's
功能
domain:The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.,function:Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.,online information:Interleukin-1 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the IL-1 family.,subcellular location:The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.,subunit:Monomer.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from ES-2 cells, using IL1B Antibody.

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,IL-1β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,IL-1β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,IL-1β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,IL-1β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Western Blot analysis of ES-2, U87, PC-3, SHSY5Y, mouse liver cells using IL-1β Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

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