BD-PT5201-IL-1β Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IL-1β Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PT5201

产品名称

IL-1β Rabbit Polyclonal Antibody

别名

IL1B; IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin

类别

常规抗体

基因名称

IL1B

蛋白名称

Interleukin-1 beta

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3553

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3553

Human Swissprot No.

P01584

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P01584/entry

Mouse Gene ID

16176

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=16176

Mouse Swissprot No.

P10749

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P10749

Rat Swissprot No.

Q63264

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q63264

免疫原

The antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range:181-230

特异性

IL-1β Polyclonal Antibody detects endogenous levels of IL-1β protein.

稀释度

WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

预测分子量

17kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008],

组织表达

Expressed in activated monocytes/macrophages (at protein level).

细胞定位

Cytoplasm, cytosol . Secreted . Lysosome . Secreted, extracellular exosome . The precursor is cytosolic (PubMed:15192144). In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted (PubMed:24201029, PubMed:33377178, PubMed:33883744). Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744). In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation (PubMed:33883744). The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10 (PubMed:32272059). .

信号通路

MAPK_ERK_Growth;MAPK_G_Protein;Cytokine-cytokine receptor interaction;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Toll_Like;NOD-like receptor;Cytosolic DNA-sensing pathway;Hematopoietic cell lineage;Type I diabetes mellitus;Alzheimer's

功能

domain:The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.,function:Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.,online information:Interleukin-1 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the IL-1 family.,subcellular location:The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.,subunit:Monomer.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from ES-2 cells, using IL1B Antibody.

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,IL-1β Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,IL-1β Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,IL-1β Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,IL-1β Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of ES-2, U87, PC-3, SHSY5Y, mouse liver cells using IL-1β Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human lung. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.