产品名称
Cdk4 Rabbit Polyclonal Antibody
别名
CDK4; Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3
蛋白名称
Cyclin-dependent kinase 4
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1019
Human Swissprot No.
P11802
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P11802/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12567
Mouse Swissprot No.
P30285
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P30285
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=94201
Rat Swissprot Link
http://www.uniprot.org/uniprot/P35426
免疫原
The antiserum was produced against synthesized peptide derived from the N-terminal region of human CDK4. AA range:1-50
特异性
Cdk4 Polyclonal Antibody detects endogenous levels of Cdk4 protein.
稀释度
IF 1:50-200 WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
cyclin dependent kinase 4(CDK4) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008],
细胞定位
Cytoplasm . Nucleus . Nucleus membrane . Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus. .
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;p53;Tight junction;T_Cell_Receptor;Pathways in cancer;Pancreatic cancer;Glioma;Melanoma;Bladder cancer;Chronic myeloid leukemia;Small cell lung cancer;Non-small cell lung cancer;
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:CDK4 mutations are involved in tumor formation.,disease:Defects in CDK4 are the cause of cutaneous malignant melanoma 3 (CMM3) [MIM:609048, 155600]. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a preexisting benign nevus, which occurs most often in the skin but also may involve other sites.,enzyme regulation:Phosphorylation at Thr-172 is necessary for enzymatic activity.,function:Probably involved in the control of the cell cycle.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Forms a stable complex with D-type G1 cyclins. Interacts with SEI1 and ZNF655/VIK.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
.jpg)
.jpg)
.jpg)
