BD-PM3136-eIF4A1(M8)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

eIF4A1(M8)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3136

产品名称

eIF4A1(M8)Mouse Monoclonal Antibody

别名

Eukaryotic initiation factor 4A-I (eIF-4A-I) (eIF4A-I) (EC 3.6.4.13) (ATP-dependent RNA helicase eIF4A-1)

类别

常规抗体

基因名称

EIF4A1

蛋白名称

Eukaryotic initiation factor 4A-I

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

1973

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1973

Human Swissprot No.

P60842

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P60842/entry

Mouse Gene ID

13681

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13681

Mouse Swissprot No.

P60843

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P60843

Rat Swissprot Link

http://www.uniprot.org/uniprot/

免疫原

Synthetic Peptide of eIF4A1

特异性

The antibody detects endogenous eIF4A1 protein.

稀释度

WB 1:1000-3000 IF 1:100-200 IHC 1:50-300

预测分子量

48kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

function:ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.,similarity:Belongs to the DEAD box helicase family.,similarity:Belongs to the DEAD box helicase family. eIF4A subfamily.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,subunit:eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and RENT2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1.,

组织表达

Bone marrow,Lung,Spleen,

细胞定位

nucleus,cytoplasm,cytosol,membrane,eukaryotic translation initiation factor 4F complex,extracellular matrix,extracellular exosome,

功能

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1,eIF4A1 Monoclonal Antibody（M8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,eIF4A1 Monoclonal Antibody（M8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,eIF4A1 Monoclonal Antibody（M8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Mouse-spleen tissue. 1,eIF4A1 Monoclonal Antibody（M8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1） 293T, 2） Hela, 3） HepG2, 4） Mouse Brain tissue,

IF analysis of Hela with antibody （Left） and DAPI （Right） diluted at 1:100.