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MK Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT5177
产品名称
MK Rabbit Polyclonal Antibody
别名
MDK; MK1; NEGF2; Midkine; MK; Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein
类别
常规抗体
基因名称
MDK
蛋白名称
Midkine
推荐应用
WB
反应种属
Human,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
4192
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4192
Human Swissprot No.
P21741
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P21741/entry
Mouse Swissprot No.
P12025
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P12025
Rat Gene ID
81517
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=81517
Rat Swissprot No.
Q9R1S9
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q9R1S9
免疫原
The antiserum was produced against synthesized peptide derived from the Internal region of human MDK. AA range:94-143
特异性
MK Polyclonal Antibody detects endogenous levels of MK protein.
稀释度
WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000.. IF 1:50-200
预测分子量
18kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012],
组织表达
Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions.
细胞定位
Secreted.
功能
function:Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.,induction:By heparin and retinoic acid.,similarity:Belongs to the pleiotrophin family.,subunit:Homodimer.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from SW480 cells, using MDK Antibody.

Western Blot analysis of SW480, MCF7, PC-3, Raji, K562, mouse eye, mouse kidney cells using MK Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human Prostate. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Prostate. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Prostate. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

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