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Amylase Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT5164
产品名称
Amylase Rabbit Polyclonal Antibody
别名
AMY1A; AMY1; AMY1B; AMY1; AMY1C; AMY1; Alpha-amylase 1; 1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; AMY2A; Pancreatic alpha-amylase; PA;1,4-alpha-D-glucan glucanohydrolase; AMY2B; Alpha-amylase 2B; 1,4-alpha-D-glucan glucanohydrolase 2B; Carcinoid alpha-amylase
类别
常规抗体
基因名称
AMY1A/AMY1B/AMY1C/AMY2A/AMY2B
蛋白名称
Alpha-amylase 1/Pancreatic alpha-amylase/Alpha-amylase 2B
推荐应用
WB
反应种属
Human,Mouse,Rat
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
276
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=276
Human Swissprot No.
P04745
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P04745/entry
Mouse Gene ID
11722
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11722
Mouse Swissprot No.
P00687
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P00687
Rat Gene ID
497039
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=497039
Rat Swissprot No.
P00689
Rat Swissprot Link
http://www.uniprot.org/uniprot/P00689
免疫原
The antiserum was produced against synthesized peptide derived from the N-terminal region of human AMY1/2. AA range:61-110
特异性
Amylase Polyclonal Antibody detects endogenous levels of Amylase protein.
稀释度
WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000.. IF 1:50-200
预测分子量
60kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],
组织表达
Glandular pool- thyroid,Saliva,Thyroid,
细胞定位
extracellular space,extracellular exosome,
信号通路
Starch and sucrose metabolism;
功能
catalytic activity:Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.,cofactor:Binds 1 calcium ion per subunit.,cofactor:Binds 1 chloride ion per subunit.,online information:Amylase entry,similarity:Belongs to the glycosyl hydrolase 13 family.,subunit:Monomer.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from HeLa cells, using AMY1/2 Antibody.

Western Blot analysis of HeLa cells using Amylase Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded mouse-liver, antibody was diluted at 1:100

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