BD-PT5155-Catalase Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Catalase Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5155

产品名称

Catalase Rabbit Polyclonal Antibody

别名

CAT; Catalase

类别

常规抗体

基因名称

CAT

蛋白名称

Catalase

推荐应用

反应种属

Human,Rat,Mouse,,Fish

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

847

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=847

Human Swissprot No.

P04040

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P04040/entry

Mouse Swissprot No.

P24270

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P24270

免疫原

The antiserum was produced against synthesized peptide derived from the C-terminal region of human CAT. AA range:478-527

特异性

Catalase Polyclonal Antibody detects endogenous levels of Catalase protein.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000. Not yet tested in other applications.

预测分子量

60kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009],

组织表达

Brain,Cajal-Retzius cell,Erythrocyte,Eye,Fibroblast,Kidney,Liver,Placenta,Platelet,Skin,Uterus,

细胞定位

Peroxisome.

信号通路

Tryptophan metabolism;Methane metabolism;Amyotrophic lateral sclerosis (ALS);

功能

catalytic activity:2 H(2)O(2) = O(2) + 2 H(2)O.,cofactor:Heme group.,cofactor:NADP.,disease:Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.,function:Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.,online information:Catalase entry,PTM:The N-terminus is blocked.,similarity:Belongs to the catalase family.,subunit:Homotetramer.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from HT29 cells, using CAT Antibody.

Immunofluorescence analysis of human-liver tissue. 1,Catalase Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-kidney-cancer tissue. 1,Catalase Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Catalase Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Catalase Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,Catalase Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of HT29, A549 cells using Catalase Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100

Western Blot analysis of 293T HELA using Catalase Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000