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Catalase Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT5155
产品名称
Catalase Rabbit Polyclonal Antibody
别名
CAT; Catalase
类别
常规抗体
基因名称
CAT
蛋白名称
Catalase
推荐应用
WB
反应种属
Human,Rat,Mouse,,Fish
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
847
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=847
Human Swissprot No.
P04040
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P04040/entry
Mouse Swissprot No.
P24270
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P24270
免疫原
The antiserum was produced against synthesized peptide derived from the C-terminal region of human CAT. AA range:478-527
特异性
Catalase Polyclonal Antibody detects endogenous levels of Catalase protein.
稀释度
IF 1:50-200 WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000. Not yet tested in other applications.
预测分子量
60kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009],
组织表达
Brain,Cajal-Retzius cell,Erythrocyte,Eye,Fibroblast,Kidney,Liver,Placenta,Platelet,Skin,Uterus,
细胞定位
Peroxisome.
信号通路
Tryptophan metabolism;Methane metabolism;Amyotrophic lateral sclerosis (ALS);
功能
catalytic activity:2 H(2)O(2) = O(2) + 2 H(2)O.,cofactor:Heme group.,cofactor:NADP.,disease:Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.,function:Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.,online information:Catalase entry,PTM:The N-terminus is blocked.,similarity:Belongs to the catalase family.,subunit:Homotetramer.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from HT29 cells, using CAT Antibody.

Immunofluorescence analysis of human-liver tissue. 1,Catalase Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-liver tissue. 1,Catalase Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-kidney-cancer tissue. 1,Catalase Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-kidney-cancer tissue. 1,Catalase Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Catalase Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,Catalase Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,Catalase Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

Western Blot analysis of HT29, A549 cells using Catalase Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100

Western Blot analysis of 293T HELA using Catalase Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

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