BD-PT5153-IL-8 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IL-8 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5153

产品名称

IL-8 Rabbit Polyclonal Antibody

别名

IL8; CXCL8; Interleukin-8; IL-8; C-X-C motif chemokine 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF;Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor

类别

常规抗体

基因名称

IL8 CXCL8

蛋白名称

Interleukin-8

推荐应用

反应种属

Human

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3576

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3576

Human Swissprot No.

P10145

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P10145/entry

免疫原

The antiserum was produced against synthesized peptide derived from the C-terminal region of human IL8. AA range:50-99

特异性

IL-8 Polyclonal Antibody detects endogenous levels of IL-8 protein.

稀释度

IF 1:50-200 WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000. Not yet tested in other applications.

预测分子量

11kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008],

组织表达

Chronic myeloid leukemia cell,Kidney,Lung,Lung carcinoma,Neutrophil,Periphe

细胞定位

Secreted.

信号通路

Cytokine-cytokine receptor interaction;Chemokine;Toll_Like;NOD-like receptor;RIG-I-like receptor;Epithelial cell signaling in Helicobacter pylori infection;Pathways in cancer;Bladder cancer;

功能

function:IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.,online information:Interleukin-8 entry,PTM:Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.,similarity:Belongs to the intercrine alpha (chemokine CxC) family.,subunit:Homodimer.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from HeLa cells, using IL8 Antibody.

Immunofluorescence analysis of human-breast-cancer tissue. 1,IL-8 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-liver-cancer tissue. 1,IL-8 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of human-kidney tissue. 1,IL-8 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of various cells using primary antibody diluted at 1:1000（4°C overnight）. Secondary antibody：Goat Anti-rabbit IgG IRDye 800（ diluted at 1:5000, 25°C, 1 hour）. Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit（SM-005, Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,IL-8 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,IL-8 Polyclonal Antibody was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western Blot analysis of HeLa cells using IL-8 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100