产品名称
PKC γ Rabbit Polyclonal Antibody
别名
PRKCG; PKCG; Protein kinase C gamma type; PKC-gamma
蛋白名称
Protein kinase C gamma type
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5582
Human Swissprot No.
P05129
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P05129/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18752
Mouse Swissprot No.
P63318
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P63318
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24681
Rat Swissprot Link
http://www.uniprot.org/uniprot/P63319
免疫原
The antiserum was produced against synthesized peptide derived from the Internal region of human PRKCG. AA range:521-570
特异性
PKC γ Polyclonal Antibody detects endogenous levels of PKC γ protein.
稀释度
WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neurop
组织表达
Expressed in Purkinje cells of the cerebellar cortex.
细胞定位
Cytoplasm . Cytoplasm, perinuclear region . Cell membrane ; Peripheral membrane protein . Cell junction, synapse, synaptosome . Cell projection, dendrite . Translocates to synaptic membranes on stimulation. .
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;ErbB_HER;Calcium;Phosphatidylinositol signaling system;Vascular smooth muscle contraction;WNT;WNT-T CELLVEGF;Focal adhesion;Tight junction;Gap junction;Natural killer cell mediated cytotoxicity;Fc gamma R-mediated phagocytos
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 3 calcium ions per subunit. The ions are bound to the C2 domain.,disease:Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.,function:This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.,online information:Retina International's Scientific Newsletter,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts with CDCP1.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.