BD-PT5138-IL-10 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IL-10 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT5138

产品名称

IL-10 Rabbit Polyclonal Antibody

别名

IL10; Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF

类别

常规抗体

基因名称

IL10

蛋白名称

Interleukin-10

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

3586

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3586

Human Swissprot No.

P22301

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P22301/entry

Mouse Swissprot No.

P18893

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P18893

免疫原

The antiserum was produced against synthesized peptide derived from the Internal region of human IL10. AA range:71-120

特异性

IL-10 Polyclonal Antibody detects endogenous levels of IL-10 protein.

稀释度

WB 1:500 - 1:2000. IHC-p: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

预测分子量

20kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011],

组织表达

Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

细胞定位

Secreted.

信号通路

Cytokine-cytokine receptor interaction;Jak_STAT;T_Cell_Receptor;Intestinal immune network for IgA production;Asthma;Autoimmune thyroid disease;Systemic lupus erythematosus;Allograft rejection;

功能

disease:Defects in IL10 are a cause of susceptibility to Crohn disease (CD) [MIM:266600]. CD is a form of remitting inflammatory bowel disease (IBD). CD may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous. Crohn disease is commonly classified as autoimmune disease.,function:Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.,online information:Interleukin-10 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the IL-10 family.,subunit:Homodimer.,tissue specificity:Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysate from THP-1 cells, using IL10 Antibody.

Western Blot analysis of THP-1, Romas, HepG2, SW480, SHSY5Y, Mouse liver cells using IL-10 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:100

Immunohistochemical analysis of paraffin-embedded Human Cerebral cortex. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human lymphoma. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.