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DNA pol ε B Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT5065
产品名称
DNA pol ε B Rabbit Polyclonal Antibody
别名
POLE2; DPE2; DNA polymerase epsilon subunit 2; DNA polymerase II subunit 2; DNA polymerase epsilon subunit B
类别
常规抗体
基因名称
POLE2
蛋白名称
DNA polymerase epsilon subunit 2
推荐应用
WB
反应种属
Human,Rat,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
5427
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5427
Human Swissprot No.
P56282
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P56282/entry
Mouse Swissprot No.
O54956
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O54956
免疫原
Synthesized peptide derived from the Internal region of human DNA pol ε B.
特异性
DNA pol ε B Polyclonal Antibody detects endogenous levels of DNA pol ε B protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
预测分子量
59kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Participates in DNA repair and in chromosomal DNA replication.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,similarity:Belongs to the DNA polymerase epsilon subunit B family.,subunit:Consists of four subunits: POLE, POLE2, POLE3 and POLE4.,
组织表达
T cells,
细胞定位
Nucleus.
信号通路
Purine metabolism;Pyrimidine metabolism;DNA replication;Base excision repair;Nucleotide excision repair;
功能
catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Participates in DNA repair and in chromosomal DNA replication.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,similarity:Belongs to the DNA polymerase epsilon subunit B family.,subunit:Consists of four subunits: POLE, POLE2, POLE3 and POLE4.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western Blot analysis of extracts from Jurkat cells, using DNA pol ε B Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

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