BD-PM3089-Oct1(7G1)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Oct1(7G1)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3089

产品名称

Oct1(7G1)Mouse Monoclonal Antibody

类别

常规抗体

基因名称

POU2F1

蛋白名称

POU domain class 2 transcription factor

推荐应用

反应种属

Human

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

5451/5452

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=5451

Human Swissprot No.

P14859/P09086

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P14859/entry

Mouse Gene ID

18986/18987

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=18986

Rat Gene ID

171068

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=171068

Rat Swissprot No.

P31503

Rat Swissprot Link

http://www.uniprot.org/uniprot/P31503

免疫原

Synthetic Peptide of 42644

特异性

The antibody detects endogenous Oct1 protein.

稀释度

WB 1:500-2000 IF 1:200 IHC 1:50-300

预测分子量

89kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010],

组织表达

Ubiquitous. Isoform 2 is lymphocyte-specific.

细胞定位

Nucleus.

功能

function:Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.,PTM:Phosphorylated by PRKDC.,similarity:Belongs to the POU transcription factor family. Class-2 subfamily.,similarity:Contains 1 homeobox DNA-binding domain.,similarity:Contains 1 POU-specific domain.,subunit:Interacts with NR3C1, AR, PGR and HCFC1.,tissue specificity:Ubiquitous. Isoform 2 is lymphocyte-specific.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,Oct1 Monoclonal Antibody（7G1） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-breast-cancer tissue. 1,Oct1 Monoclonal Antibody（7G1）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1） Hela, 2） Jurkat, 3） HepG2, diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.