产品名称
Gl Syn Rabbit Polyclonal Antibody
别名
GLUL; GLNS; Glutamine synthetase; GS; Glutamate decarboxylase; Glutamate--ammonia ligase
蛋白名称
Glutamine synthetase
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2752
Human Swissprot No.
P15104
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P15104/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14645
Mouse Swissprot No.
P15105
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P15105
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24957
Rat Swissprot Link
http://www.uniprot.org/uniprot/P09606
免疫原
The antiserum was produced against synthesized peptide derived from human Gl Syn. AA range:295-344
特异性
Gl Syn Polyclonal Antibody detects endogenous levels of Gl Syn protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],
组织表达
Expressed in endothelial cells.
细胞定位
Cytoplasm, cytosol . Microsome . Mitochondrion . Cell membrane ; Lipid-anchor . Mainly localizes in the cytosol, with a fraction associated with the cell membrane. .
信号通路
Alanine; aspartate and glutamate metabolism;Arginine and proline metabolism;Nitrogen metabolism;
功能
catalytic activity:ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine.,disease:Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.,online information:Glutamine synthetase entry,similarity:Belongs to the glutamine synthetase family.,subunit:Homooctamer.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
