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CHD1L Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT5027
产品名称
CHD1L Rabbit Polyclonal Antibody
别名
CHD1L; ALC1; Chromodomain-helicase-DNA-binding protein 1-like; Amplified in liver cancer protein 1
类别
常规抗体
基因名称
CHD1L
蛋白名称
Chromodomain-helicase-DNA-binding protein 1-like
推荐应用
WB
反应种属
Human,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
9557
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9557
Human Swissprot No.
Q86WJ1
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q86WJ1/entry
Mouse Gene ID
68058
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=68058
Mouse Swissprot No.
Q9CXF7
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9CXF7
免疫原
The antiserum was produced against synthesized peptide derived from human CHD1L. AA range:545-594
特异性
CHD1L Polyclonal Antibody detects endogenous levels of CHD1L protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
预测分子量
101kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
chromodomain helicase DNA binding protein 1 like(CHD1L) Homo sapiens This gene encodes a DNA helicase protein involved in DNA repair. The protein converts ATP to add poly(ADP-ribose) as it regulates chromatin relaxation following DNA damage. Several alternatively spliced transcripts variants have been described for this gene. [provided by RefSeq, Jan 2012],
组织表达
Frequently overexpressed in hepatomacellular carcinomas.
细胞定位
Nucleus . Localizes at sites of DNA damage. Probably recruited to DNA damage sites by PARylated PARP1. .
功能
function:Putative DNA helicase, which may play a role in chromatin-remodeling. In vitro, promotes cell proliferation and inhibits apoptois.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 1 Macro domain.,tissue specificity:Frequently overexpresssed in hepatomacellular carcinomas.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from HepG2 cells, using CHD1L antibody.

Western Blot analysis of extracts from NIH-3T3, Jurkat, A549 cells, using CHD1L Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

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