产品名称
V-ATPase D1 Rabbit Polyclonal Antibody
别名
ATP6V0D1; ATP6D; VPATPD; V-type proton ATPase subunit d 1; V-ATPase subunit d 1; 32 kDa accessory protein; V-ATPase 40 kDa accessory protein; V-ATPase AC39 subunit; p39; Vacuolar proton pump subunit d 1
蛋白名称
V-type proton ATPase subunit d 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9114
Human Swissprot No.
P61421
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P61421/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11972
Mouse Swissprot No.
P51863
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P51863
免疫原
The antiserum was produced against synthesized peptide derived from human V-ATPase D1. AA range:221-270
特异性
V-ATPase D1 Polyclonal Antibody detects endogenous levels of V-ATPase D1 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [pro
细胞定位
Membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome membrane ; Peripheral membrane protein . Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein . Localizes to centrosome and the base of the cilium. .
信号通路
Oxidative phosphorylation;Lysosome;Vibrio cholerae infection;Epithelial cell signaling in Helicobacter pylori infection;
功能
function:Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis.,similarity:Belongs to the V-ATPase V0D/AC39 subunit family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d).,tissue specificity:Ubiquitous.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.