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β-1,4-Gal-T5 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT5010
产品名称
β-1,4-Gal-T5 Rabbit Polyclonal Antibody
别名
B4GALT5; Beta-1; 4-galactosyltransferase 5; Beta-1,4-GalTase 5; Beta4Gal-T5; b4Gal-T5; Beta-1,4-GalT II; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5
类别
常规抗体
基因名称
B4GALT5
蛋白名称
Beta-1,4-galactosyltransferase 5
推荐应用
WB
反应种属
Human,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
9334
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9334
Human Swissprot No.
O43286
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O43286/entry
Mouse Gene ID
56336
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=56336
Mouse Swissprot No.
Q9JMK0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9JMK0
免疫原
The antiserum was produced against synthesized peptide derived from human B4GALT5. AA range:321-370
特异性
β-1,4-Gal-T5 Polyclonal Antibody detects endogenous levels of β-1,4-Gal-T5 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
预测分子量
40kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was previously designated as B4GALT4 but was renamed
组织表达
Ubiquitously expressed.
细胞定位
Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein. Golgi apparatus . Trans cisternae of Golgi stack. .
信号通路
O-Glycan biosynthesis;
功能
cofactor:Manganese.,function:Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.,online information:Beta-1,4-galactosyltransferase 5,online information:GlycoGene database,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 7 family.,subcellular location:Trans cisternae of Golgi stack.,tissue specificity:Ubiquitously expressed.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western Blot analysis of various cells using β-1,4-Gal-T5 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Western Blot analysis of 293 cells using β-1,4-Gal-T5 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

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