产品名称
XRCC4(5C10)Mouse Monoclonal Antibody
别名
XRCC4; DNA repair protein XRCC4; X-ray repair cross-complementing protein 4
蛋白名称
DNA repair protein XRCC4
存储缓冲液
PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7518
Human Swissprot No.
Q13426
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q13426/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=108138
Mouse Swissprot No.
Q924T3
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q924T3
Rat Swissprot Link
http://www.uniprot.org/uniprot/
免疫原
Synthetic Peptide of XRCC4
特异性
The antibody detects endogenous XRCC4 proteins.
稀释度
WB 1:2000 IP:1:200 IF 1:200 IHC 1:50-300
背景介绍
The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants. [provided by RefSeq, Dec 2015],
细胞定位
Nucleus . Chromosome . Localizes to site of double-strand breaks. .; [Protein XRCC4, C-terminus]: Cytoplasm . Translocates from the nucleus to the cytoplasm following cleavage by caspase-3 (CASP3). .
信号通路
Non-homologous end-joining;
功能
function:Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.,PTM:Monoubiquitinated.,PTM:Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.,PTM:Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.,similarity:Belongs to the XRCC4 family.,subunit:Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC. Seems to interact directly with PRKDC but not with the Ku p70/86 dimer. Interacts with XLF/Cernunnos. Interacts with APTX and APLF.,tissue specificity:Widely expressed.,
纯化
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
