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α-protein Kinase 1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT5001
产品名称
α-protein Kinase 1 Rabbit Polyclonal Antibody
别名
ALPK1; KIAA1527; LAK; Alpha-protein kinase 1; Chromosome 4 kinase; Lymphocyte alpha-protein kinase
类别
常规抗体
基因名称
ALPK1
蛋白名称
Alpha-protein kinase 1
推荐应用
WB
反应种属
Human,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
80216
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=80216
Human Swissprot No.
Q96QP1
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q96QP1/entry
Mouse Swissprot No.
Q9CXB8
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9CXB8
免疫原
The antiserum was produced against synthesized peptide derived from human ALPK1. AA range:11-60
特异性
α-protein Kinase 1 Polyclonal Antibody detects endogenous levels of α-protein Kinase 1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
预测分子量
139kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects (PMID: 21208416). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011],
组织表达
Highly expressed in liver. Expressed in the optic nerve and retinal pigmented epithelium. Lower expression is observed in the macula and extramacular retina (PubMed:30967659).
细胞定位
Cytoplasm, cytosol . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cell projection, cilium . Localized at the base of primary cilia. .
功能
function:Kinases that recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.,similarity:Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.,similarity:Contains 1 alpha-type protein kinase domain.,tissue specificity:Highly expressed in liver.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from COLO and HepG2 cells, using ALPK1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from 293 cells using ALPK1 antibody.

Western Blot analysis of various cells using α-protein Kinase 1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

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