产品名称
ZP4 Rabbit Polyclonal Antibody
别名
ZP4; ZPB; Zona pellucida sperm-binding protein 4; Zona pellucida glycoprotein 4; Zp-4; Zona pellucida protein B
蛋白名称
Zona pellucida sperm-binding protein 4
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=57829
Human Swissprot No.
Q12836
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q12836/entry
免疫原
The antiserum was produced against synthesized peptide derived from human ZP4. AA range:231-280
特异性
ZP4 Polyclonal Antibody detects endogenous levels of ZP4 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been
组织表达
Expressed in oocytes.
细胞定位
[Processed zona pellucida sperm-binding protein 4]: Zona pellucida .; Cell membrane ; Single-pass type I membrane protein .
功能
domain:The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.,function:The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor.,PTM:Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.,similarity:Belongs to the ZP domain family. ZPB subfamily.,similarity:Contains 1 P-type (trefoil) domain.,similarity:Contains 1 ZP domain.,tissue specificity:Oocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
