BD-PM3085-EFHD1(3G2)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

EFHD1(3G2)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3085

产品名称

EFHD1(3G2)Mouse Monoclonal Antibody

别名

EF-hand domain-containing protein D1 (EF-hand domain-containing protein 1) (Swiprosin-2)

类别

常规抗体

基因名称

EFHD1

蛋白名称

EF-hand domain-containing protein D1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

80303

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=80303

Human Swissprot No.

Q9BUP0

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q9BUP0/entry

Mouse Gene ID

98363

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=98363

Mouse Swissprot No.

Q9D4J1

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q9D4J1

Rat Swissprot Link

http://www.uniprot.org/uniprot/

免疫原

Synthetic Peptide of EFHD1

特异性

The antibody detects endogenous EFHD1 proteins.

稀释度

WB 1:2000 IF 1:100-200 IHC 1:50-300

预测分子量

27kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes a member of the EF-hand super family of calcium binding proteins, which are involved in a variety of cellular processes including mitosis, synaptic transmission, and cytoskeletal rearrangement. The protein encoded by this gene is composed of an N-terminal disordered region, proline-rich elements, two EF-hands, and a C-terminal coiled-coil domain. This protein has been shown to associate with the mitochondrial inner membrane, and in HeLa cells, acts as a novel mitochondrial calcium ion sensor for mitochondrial flash activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016],

组织表达

Brain,Eye,Heart,Hippocampus,Lung,Normal aorta,Placenta,

细胞定位

Mitochondrion inner membrane .

功能

similarity:Contains 2 EF-hand domains.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,EFHD1 Monoclonal Antibody（3G2） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,EFHD1 Monoclonal Antibody（3G2） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,EFHD1 Monoclonal Antibody（3G2） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Mouse-lung tissue. 1,EFHD1 Monoclonal Antibody（3G2）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1） Mouse spleen tissue, 2） Rat spleen tissue, diluted at 1:3000.

IF analysis of Hela with antibody （Left） and DAPI （Right） diluted at 1:100.