产品名称
ZnT-8 Rabbit Polyclonal Antibody
别名
SLC30A8; ZNT8; Zinc transporter 8; ZnT-8; Solute carrier family 30 member 8
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=169026
Human Swissprot No.
Q8IWU4
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q8IWU4/entry
Mouse Swissprot No.
Q8BGG0
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8BGG0
免疫原
The antiserum was produced against synthesized peptide derived from human SLC30A8. AA range:171-220
特异性
ZnT-8 Polyclonal Antibody detects endogenous levels of ZnT-8 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles. This gene is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The encoded protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent (NIDDM). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010],
组织表达
In the endocrine pancreas, expressed in insulin-producing beta cells. Expressed at relatively high levels in subcutaneous fat tissue from lean persons; much lower levels in visceral fat, whether from lean or obese individuals, and in subcutaneous fat tissue from obese individuals. Expressed in peripheral blood mononuclear cells, including T-cells and B-cells, with great variation among individuals ranging from negative to strongly positive.
细胞定位
Cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, secretory vesicle membrane ; Multi-pass membrane protein . Associated with insulin and glucagon secretory granules. .
功能
domain:Contains a histidine-rich region, HXXXXXHNH-motif, which is a ligand for zinc.,function:Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta-cells.,polymorphism:Variant Trp-325 is a risk factor that confers susceptibility to diabetes mellitus, noninsulin-dependent (NIDDM) [MIM:125853].,similarity:Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.,subcellular location:Strictly associated with the insulin granule secretory.,tissue specificity:Strictly restricted to the pancreas islets cells. Found in insulin-producing beta cells and colocalized with insulin.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
