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ZNF420 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PT4965
产品名称
ZNF420 Rabbit Polyclonal Antibody
别名
ZNF420; Zinc finger protein 420
类别
常规抗体
基因名称
ZNF420
蛋白名称
Zinc finger protein 420
推荐应用
WB
反应种属
Human,Mouse
浓度
1 mg/ml
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene ID
147923
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=147923
Human Swissprot No.
Q8TAQ5
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q8TAQ5/entry
免疫原
The antiserum was produced against synthesized peptide derived from human ZNF420. AA range:301-350
特异性
ZNF420 Polyclonal Antibody detects endogenous levels of ZNF420 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
预测分子量
80kD
运输及保存条件
-20°C/1 year
宿主
Polyclonal, Rabbit,IgG
背景介绍
zinc finger protein 420(ZNF420) Homo sapiens The protein encoded by this gene is a KRAB-type zinc finger protein that negatively-regulates p53-mediated apoptosis. Under stress conditions, the encoded protein is phosphorylated by ATM and dissociates from p53, which activates p53 and initiates apoptosis. [provided by RefSeq, Jul 2016],
组织表达
Muscle,Placenta,Testis,
细胞定位
Nucleus .
功能
function:May be involved in transcriptional regulation.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 KRAB domain.,similarity:Contains 19 C2H2-type zinc fingers.,
期货
现货
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of ZNF420 Antibody. The lane on the right is blocked with the ZNF420 peptide.

Western blot analysis of the lysates from COLO205 cells using ZNF420 antibody.

Western Blot analysis of K562 cells using ZNF420 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

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