BD-PM3076-CA IX(12F10)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CA IX(12F10)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3076

产品名称

CA IX(12F10)Mouse Monoclonal Antibody

别名

CA9; G250; MN; Carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1

类别

常规抗体

基因名称

CA9

蛋白名称

Carbonic anhydrase 9

推荐应用

反应种属

Human

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

768

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=768

Human Swissprot No.

Q16790

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q16790/entry

Mouse Gene ID

230099

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=230099

Mouse Swissprot No.

Q8VHB5

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q8VHB5

Rat Swissprot Link

http://www.uniprot.org/uniprot/

免疫原

Synthetic Peptide of CA IX

特异性

The antibody detects endogenous CA IX proteins.

稀释度

IF 1:50-200 WB 1:3000 IP:1:200 IHC 1:50-300

预测分子量

38-48kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014],

组织表达

Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

细胞定位

Nucleus . Nucleus, nucleolus . Cell membrane ; Single-pass type I membrane protein . Cell projection, microvillus membrane ; Single-pass type I membrane protein . Found on the surface microvilli and in the nucleus, particularly in nucleolus.

信号通路

Nitrogen metabolism;

功能

catalytic activity:H(2)CO(3) = CO(2) + H(2)O.,cofactor:Zinc.,function:Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.,induction:By hypoxia.,PTM:Asn-346 bears high-mannose type glycan structures.,similarity:Belongs to the alpha-carbonic anhydrase family.,subcellular location:Found on the surface microvilli and in the nucleus, particularly in nucleolus.,subunit:Forms oligomers linked by disulfide bonds.,tissue specificity:Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunofluorescence analysis of human-liver-cancer tissue. 1,CA IX Monoclonal Antibody（12F10）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CA IX Monoclonal Antibody（12F10） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Western blot analysis of 1） Hela, 2） 293T, diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

1） Input: Hela Cell Lysate 2） IP product: IP dilute 1:200

Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.