BD-PM3075-AFP alpha 1 Fetoprotein(17C5)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

AFP alpha 1 Fetoprotein(17C5)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3075

产品名称

AFP alpha 1 Fetoprotein(17C5)Mouse Monoclonal Antibody

别名

AFP; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

类别

常规抗体

基因名称

AFP

蛋白名称

Alpha-fetoprotein

推荐应用

反应种属

Human

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

174

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=174

Human Swissprot No.

P02771

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P02771/entry

Mouse Gene ID

11576

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11576

Mouse Swissprot No.

P02772

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P02772

Rat Swissprot No.

P02773

Rat Swissprot Link

http://www.uniprot.org/uniprot/P02773

免疫原

Synthetic Peptide of AFP alpha 1 Fetoprotein

特异性

The antibody detects endogenous AFP proteins.

稀释度

WB 1:2000 IF 1:200 IHC 1:50-300

预测分子量

70kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008],

组织表达

Plasma. Synthesized by the fetal liver and yolk sac.

细胞定位

Secreted.

功能

developmental stage:Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.,function:Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.,online information:Alpha-fetoprotein entry,PTM:Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.,PTM:Sulfated.,similarity:Belongs to the ALB/AFP/VDB family.,similarity:Contains 3 albumin domains.,subunit:Dimeric and trimeric forms have been found in addition to the monomeric form.,tissue specificity:Plasma. Synthesized by the fetal liver and yolk sac.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,AFP alpha 1 Fetoprotein Monoclonal Antibody（17C5） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-breast-cancer tissue. 1,AFP alpha 1 Fetoprotein Monoclonal Antibody（17C5）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of HepG2, diluted at 1:2,000.

Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human Prostate. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.