BD-PT4913-XIAP Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

XIAP Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4913

产品名称

XIAP Rabbit Polyclonal Antibody

别名

XIAP; API3; BIRC4; IAP3; E3 ubiquitin-protein ligase XIAP; Baculoviral IAP repeat-containing protein 4; IAP-like protein; ILP; hILP; Inhibitor of apoptosis protein 3; IAP-3; hIAP-3; hIAP3; X-linked inhibitor of apoptosis protein; X-linked I

类别

常规抗体

基因名称

XIAP

蛋白名称

E3 ubiquitin-protein ligase XIAP

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

331

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=331

Human Swissprot No.

P98170

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P98170/entry

Mouse Gene ID

11798

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11798

Mouse Swissprot No.

Q60989

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q60989

Rat Gene ID

63879

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=63879

Rat Swissprot No.

Q9R0I6

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q9R0I6

免疫原

The antiserum was produced against synthesized peptide derived from human XIAP. AA range:53-102

特异性

XIAP Polyclonal Antibody detects endogenous levels of XIAP protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

57kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011],

组织表达

Expressed in colonic crypts (at protein level) (PubMed:30389919). Ubiquitous, except peripheral blood leukocytes (PubMed:8654366).

细胞定位

Cytoplasm. Nucleus. TLE3 promotes its nuclear localization.

信号通路

Ubiquitin mediated proteolysis;Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Focal adhesion;NOD-like receptor;Pathways in cancer;Small cell lung cancer;

功能

disease:Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.,domain:The first BIR domain is involved in interaction with MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.,function:Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.,online information:XIAP mutation db,PTM:Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.,PTM:Ubiquitinated and degraded by the proteasome in apoptotic cells.,similarity:Belongs to the IAP family.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 3 BIR repeats.,subunit:Monomer, and homodimer. Interacts with SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with MAP3K7IP1 and AIFM1. Interaction with SMAC hinders binding of MAP3K7IP1 and AIFM1. Interacts with TCF25.,tissue specificity:Ubiquitous, except peripheral blood leukocytes.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using XIAP Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using XIAP Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using XIAP Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Western Blot analysis of 293 cells using XIAP Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.