产品名称
WTAP Rabbit Polyclonal Antibody
别名
WTAP; KIAA0105; Pre-mRNA-splicing regulator WTAP; Female-lethal(2)D homolog; hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein
蛋白名称
Pre-mRNA-splicing regulator WTAP
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=9589
Human Swissprot No.
Q15007
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q15007/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=60532
Mouse Swissprot No.
Q9ER69
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9ER69
免疫原
The antiserum was produced against synthesized peptide derived from human WTAP. AA range:321-370
特异性
WTAP Polyclonal Antibody detects endogenous levels of WTAP protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012],
组织表达
Ubiquitously expressed.
细胞定位
Nucleus speckle . Nucleus, nucleoplasm . Cytoplasm . Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus. .
功能
function:Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.,induction:In smooth muscle cells, up-regulated after serum withdrawal, when cells become mature and non proliferative.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the fl(2)d family.,subunit:Interacts with WT1.,tissue specificity:Ubiquitously expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
