BD-PM3067-MAP2(7D4)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MAP2(7D4)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3067

产品名称

MAP2(7D4)Mouse Monoclonal Antibody

别名

MAP2; Microtubule-associated protein 2; MAP-2

类别

常规抗体

基因名称

MAP2

蛋白名称

Microtubule-associated protein 2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

4133

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4133

Human Swissprot No.

P11137

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P11137/entry

Mouse Swissprot No.

P20357

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P20357

Rat Swissprot No.

P15146

Rat Swissprot Link

http://www.uniprot.org/uniprot/P15146

免疫原

Synthetic Peptide of MAP2

特异性

The antibody detects endogenous MAP2 proteins.

稀释度

WB 500-2000 1:200 IF 1:50-200

参考分子量

200kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010],

组织表达

Brain,Brain cortex,Epithelium,Pancreas,Testis,

细胞定位

Cytoplasm, cytoskeleton . Cell projection, dendrite .

功能

alternative products:Additional isoforms seem to exist,function:The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.,PTM:MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 3 Tau/MAP repeats.,similarity:Contains 4 Tau/MAP repeats.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,MAP2 Monoclonal Antibody（7D4） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,MAP2 Monoclonal Antibody（7D4） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,MAP2 Monoclonal Antibody（7D4） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Mouse-brain tissue. 1,MAP2 Monoclonal Antibody（7D4）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-brain tissue. 1,MAP2 Monoclonal Antibody（7D4）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

IHC staining of Human brain tissue paraffin-embedded, diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:400（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.