BD-PM3066-NSE(13E2)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

NSE(13E2)Mouse Monoclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PM3066

产品名称

NSE(13E2)Mouse Monoclonal Antibody

别名

ENO2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

类别

常规抗体

基因名称

ENO2

蛋白名称

Gamma-enolase

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

2026

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2026

Human Swissprot No.

P09104

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P09104/entry

Mouse Gene ID

13807

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=13807

Mouse Swissprot No.

P17183

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P17183

Rat Gene ID

100911625

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100911625

Rat Swissprot No.

P07323

Rat Swissprot Link

http://www.uniprot.org/uniprot/P07323

免疫原

Synthetic Peptide of NSE

特异性

The antibody detects endogenous NSE proteins.

稀释度

WB 1:2000 IHC 1:200 IF 1:200

预测分子量

47kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

enolase 2(ENO2) Homo sapiens This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008],

组织表达

The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

细胞定位

Cytoplasm . Cell membrane . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. .

信号通路

Glycolysis / Gluconeogenesis;RNA degradation;

功能

catalytic activity:2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O.,cofactor:Magnesium. Required for catalysis and for stabilizing the dimer.,developmental stage:During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.,function:Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.,induction:Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jacob disease.,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.,similarity:Belongs to the enolase family.,subcellular location:Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.,subunit:Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.,tissue specificity:The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,NSE Monoclonal Antibody（13E2） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,NSE Monoclonal Antibody（13E2） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,NSE Monoclonal Antibody（13E2） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-appendix tissue. 1,NSE Monoclonal Antibody（13E2）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-spleen tissue. 1,NSE Monoclonal Antibody（13E2）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1） Hela, 2） Jurkat, 3） 293T cell lysates, diluted at 1:3000.

IHC staining of Human small cell carcinoma of lung tissue, diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:400（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.