BD-PM3064-Ki 67(4A8)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Ki 67(4A8)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3064

产品名称

Ki 67(4A8)Mouse Monoclonal Antibody

别名

MKI67; Antigen KI-67

类别

常规抗体

基因名称

MKI67

蛋白名称

Antigen KI-67

推荐应用

反应种属

Human

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

4288

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=4288

Human Swissprot No.

P46013

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P46013/entry

免疫原

Synthetic Peptide of Ki 67

特异性

The antibody detects endogenous Ki 67 proteins.

稀释度

IHC 1:200 IF 1:50-200

参考分子量

359kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009],

组织表达

Epithelium,

细胞定位

Chromosome . Nucleus . Nucleus, nucleolus . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). .

功能

developmental stage:Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.,function:Thought to be required for maintaining cell proliferation.,online information:Ki-67 entry,similarity:Contains 1 FHA domain.,subcellular location:Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.,subunit:Interacts with KIF15. Binds through the FHA domain to MKI67IP.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,Ki 67 Monoclonal Antibody（4A8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,Ki 67 Monoclonal Antibody（4A8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-breast-cancer tissue. 1,Ki 67 Monoclonal Antibody（4A8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-testis tissue. 1,Ki 67 Monoclonal Antibody（4A8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-brain tissue. 1,Ki 67 Monoclonal Antibody（4A8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

IHC staining of Mouse Kidney tissue, diluted at 1:200.