BD-PM3062-Aquaporin 4(4H1)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Aquaporin 4(4H1)Mouse Monoclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PM3062

产品名称

Aquaporin 4(4H1)Mouse Monoclonal Antibody

别名

AQP4; Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4

类别

常规抗体

基因名称

AQP4

蛋白名称

Aquaporin-4

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

361

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=361

Human Swissprot No.

P55087

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P55087/entry

Mouse Gene ID

11829

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=11829

Mouse Swissprot No.

P55088

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P55088

Rat Gene ID

25293

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=25293

Rat Swissprot No.

P47863

Rat Swissprot Link

http://www.uniprot.org/uniprot/P47863

免疫原

Synthetic Peptide of Aquaporin 4

特异性

The antibody detects endogenous Aquaporin 4 proteins.

稀释度

WB 1:1000 IF 1:100-200 IHC 1:50-300

预测分子量

48kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015],

组织表达

Detected in skeletal muscle (PubMed:29055082). Detected in stomach, along the glandular base region of the fundic gland (at protein level) (PubMed:8601457). Detected in brain, lung and skeletal muscle, and at much lower levels in heart and ovary (PubMed:7559426, PubMed:8601457).

细胞定位

Cell membrane ; Multi-pass membrane protein . Basolateral cell membrane ; Multi-pass membrane protein . Endosome membrane . Cell membrane, sarcolemma ; Multi-pass membrane protein . Cell projection . Activation of the vasopressin receptor AVPR1A triggers AQP4 phosphorylation at Ser-180 and promotes its internalization from the cell membrane. Detected on brain astrocyte processes and astrocyte endfeet close to capillaries. .

功能

domain:Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).,function:Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.,similarity:Belongs to the MIP/aquaporin (TC 1.A.8) family.,tissue specificity:Brain - muscle >> heart, kidney, lung, and trachea.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Aquaporin 4 Monoclonal Antibody（4H1） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-appendix tissue. 1,Aquaporin 4 Monoclonal Antibody（4H1）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-brain tissue. 1,Aquaporin 4 Monoclonal Antibody（4H1）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1） Hela, 2） Mouse Heart tissue, 3） Rat Heart Tissue, diluted at 1:2000.

IF analysis of Hela with antibody （Left） and DAPI （Right） diluted at 1:100.