BD-PT4841-USP44 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

USP44 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4841

产品名称

USP44 Rabbit Polyclonal Antibody

别名

USP44; Ubiquitin carboxyl-terminal hydrolase 44; Deubiquitinating enzyme 44; Ubiquitin thioesterase 44; Ubiquitin-specific-processing protease 44

类别

常规抗体

基因名称

USP44

蛋白名称

Ubiquitin carboxyl-terminal hydrolase 44

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

84101

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=84101

Human Swissprot No.

Q9H0E7

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q9H0E7/entry

Mouse Swissprot No.

Q8C2S0

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q8C2S0

免疫原

The antiserum was produced against synthesized peptide derived from human USP44. AA range:211-260

特异性

USP44 Polyclonal Antibody detects endogenous levels of USP44 protein.

稀释度

WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

81kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP44 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM, Mar 2008],

组织表达

Expressed in testis. Expressed at high levels in T-cell acute lymphoblastic leukemia.

细胞定位

Nucleus . Peaks in interphase, with relatively low levels maintained throughout mitosis. .

功能

catalytic activity:Ubiquitin C-terminal thioester + H(2)O = ubiquitin + a thiol.,similarity:Belongs to the peptidase C19 family.,similarity:Contains 1 UBP-type zinc finger.,tissue specificity:Expressed in testis.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from LOVO and NIH/3T3 cells, using USP44 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of 293T cells using USP44 Polyclonal Antibody diluted at 1：2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western blot analysis of mouse-kidney lysis using USP44 antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.