BD-PM3059-GFAP(5C8)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GFAP(5C8)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3059

产品名称

GFAP(5C8)Mouse Monoclonal Antibody

别名

GFAP; Glial fibrillary acidic protein; GFAP

类别

常规抗体

基因名称

GFAP

蛋白名称

Glial fibrillary acidic protein

推荐应用

反应种属

Human,Rat,Mouse

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

2670

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2670

Human Swissprot No.

P14136

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P14136/entry

Mouse Gene ID

14580

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=14580

Mouse Swissprot No.

P03995

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P03995

Rat Gene ID

24387

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24387

Rat Swissprot No.

P47819

Rat Swissprot Link

http://www.uniprot.org/uniprot/P47819

免疫原

Synthetic Peptide of GFAP

特异性

The antibody detects endogenous GFAP proteins.

稀释度

WB 1:2000-5000 IF 1:200 IHC 1:50-300

预测分子量

45kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008],

组织表达

Expressed in cells lacking fibronectin.

细胞定位

Cytoplasm . Associated with intermediate filaments. .

功能

alternative products:Isoforms differ in the C-terminal region which is encoded by alternative exons,disease:Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.,function:GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.,online information:GFAP entry,similarity:Belongs to the intermediate filament family.,subcellular location:Associated with intermediate filaments.,subunit:Interacts with SYNM (By similarity). Isoform 3 interacts with PSEN1 (via N-terminus).,tissue specificity:Expressed in cells lacking fibronectin.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,GFAP Monoclonal Antibody（5C8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,GFAP Monoclonal Antibody（5C8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,GFAP Monoclonal Antibody（5C8） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Mouse-brain tissue. 1,GFAP Monoclonal Antibody（5C8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-brain tissue. 1,GFAP Monoclonal Antibody（5C8）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of Rat Brain Tissue, diluted at 1:5000.