BD-PT4827-USF-2 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

USF-2 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4827

产品名称

USF-2 Rabbit Polyclonal Antibody

别名

USF2; BHLHB12; Upstream stimulatory factor 2; Class B basic helix-loop-helix protein 12; bHLHb12; FOS-interacting protein; FIP; Major late transcription factor 2; Upstream transcription factor 2

类别

常规抗体

基因名称

USF2

蛋白名称

Upstream stimulatory factor 2

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

7392

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7392

Human Swissprot No.

Q15853

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q15853/entry

Mouse Gene ID

22282

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=22282

Mouse Swissprot No.

Q64705

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q64705

Rat Gene ID

81817

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=81817

Rat Swissprot No.

Q63665

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q63665

免疫原

The antiserum was produced against synthesized peptide derived from human USF2. AA range:196-245

特异性

USF-2 Polyclonal Antibody detects endogenous levels of USF-2 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

预测分子量

40kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the basic helix-loop-helix leucine zipper family of transcription factors. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs and is involved in regulating multiple cellular processes. [provided by RefSeq, Mar 2016],

组织表达

Ubiquitous.

细胞定位

Nucleus.

功能

alternative products:Additional isoforms seem to exist,function:Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Interacts with MAF (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an homodimer or a heterodimer (USF1/USF2). In vivo, the USF1/USF2A heterodimer represents over 66% of the usf binding activity whereas the USF1 and USF2A homodimers represent less than 10%. The USF1/USF2B heterodimer accounted for almost 15% in some cell.,tissue specificity:Ubiquitous.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using USF2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, using USF2 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using USF-2 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of HeLa cells using USF-2 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.