BD-PM3057-CDX2(14H6)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CDX2(14H6)Mouse Monoclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PM3057

产品名称

CDX2(14H6)Mouse Monoclonal Antibody

别名

CDX2; CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2

类别

常规抗体

基因名称

CDX2

蛋白名称

Homeobox protein CDX-2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

1045

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1045

Human Swissprot No.

Q99626

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q99626/entry

Mouse Gene ID

12591

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12591

Mouse Swissprot No.

P43241

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P43241

Rat Swissprot Link

http://www.uniprot.org/uniprot/

免疫原

Synthetic Peptide of CDX2

特异性

The antibody detects endogenous CDX2 proteins.

稀释度

WB 1:1000 IHC 1:200 IF 1:200

预测分子量

42kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012],

组织表达

Detected in small intestine, colon and pancreas.

细胞定位

Nucleus .

功能

function:Involved in the transciptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.,PTM:Phosphorylation of Ser-60 mediates the transactivation capacity.,similarity:Belongs to the Caudal homeobox family.,similarity:Contains 1 homeobox DNA-binding domain.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,CDX2 Monoclonal Antibody（14H6） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,CDX2 Monoclonal Antibody（14H6） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,CDX2 Monoclonal Antibody（14H6） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Mouse-kidney tissue. 1,CDX2 Monoclonal Antibody（14H6）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-spleen tissue. 1,CDX2 Monoclonal Antibody（14H6）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1） 293T, 2） Mouse Heart tissue, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

IHC staining of human rectal cancer tissue, diluted at 1:200.