产品名称
UBE3B Rabbit Polyclonal Antibody
别名
UBE3B; Ubiquitin-protein ligase E3B
蛋白名称
Ubiquitin-protein ligase E3B
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=89910
Human Swissprot No.
Q7Z3V4
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q7Z3V4/entry
Mouse Swissprot No.
Q9ES34
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9ES34
免疫原
The antiserum was produced against synthesized peptide derived from human UBE3B. AA range:581-630
特异性
UBE3B Polyclonal Antibody detects endogenous levels of UBE3B protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [pro
信号通路
Ubiquitin mediated proteolysis;
功能
function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.,similarity:Contains 1 IQ domain.,tissue specificity:Widely expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
