产品名称
UBE1L Rabbit Polyclonal Antibody
别名
UBA7; UBE1L; UBE2; Ubiquitin-like modifier-activating enzyme 7; Ubiquitin-activating enzyme 7; D8; Ubiquitin-activating enzyme E1 homolog
蛋白名称
Ubiquitin-like modifier-activating enzyme 7
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7318
Human Swissprot No.
P41226
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P41226/entry
免疫原
The antiserum was produced against synthesized peptide derived from human UBE1L. AA range:963-1012
特异性
UBE1L Polyclonal Antibody detects endogenous levels of UBE1L protein.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008],
组织表达
Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
细胞定位
nucleus,nucleoplasm,cytosol,
信号通路
Ubiquitin mediated proteolysis;Parkinson's disease;
功能
function:Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.,miscellaneous:There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-activating E1 family.,subunit:Monomer (By similarity). Binds and is involved in the conjugation of G1P2/ISG15.,tissue specificity:Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
