BD-PM3042-HSP70(3G10)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HSP70(3G10)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3042

产品名称

HSP70(3G10)Mouse Monoclonal Antibody

类别

常规抗体

基因名称

HSPA1L/HSPA1A

蛋白名称

Heat shock 70 kDa protein 1-like/Heat shock 70 kDa protein 1A/1B

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

3305/3303/3304

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=3305

Human Swissprot No.

P34931/P08107

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P34931/entry

Mouse Gene ID

15482/193740

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=15482

Rat Gene ID

24963/24472

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24963

Rat Swissprot No.

P55063/Q07439

Rat Swissprot Link

http://www.uniprot.org/uniprot/P55063

免疫原

Synthetic Peptide of HSP70

特异性

The antibody detects endogenous HSP70 proteins.

稀释度

WB 1:1000-2000 IF 1:100-200 IHC 1:50-300

预测分子量

70kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq, Jul 2008],

组织表达

Expressed in spermatids.

细胞定位

zona pellucida receptor complex,nucleoplasm,mitochondrial matrix,cytosol,COP9 signalosome,cell body,blood microparticle,

信号通路

Spliceosome;MAPK_ERK_Growth;MAPK_G_Protein;Endocytosis;Antigen processing and presentation;

功能

function:In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.,induction:Not induced by heat shock.,similarity:Belongs to the heat shock protein 70 family.,tissue specificity:Expressed in spermatids.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,HSP70 Monoclonal Antibody（3G10） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,HSP70 Monoclonal Antibody（3G10） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,HSP70 Monoclonal Antibody（3G10） was diluted at 1:200（4°C,overnight）. 2, Sodium citrate pH 6.0 was used for antibody retrieval（>98°C,20min）. 3,Secondary antibody was diluted at 1:200（room tempeRature, 30min）. Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-breast-cancer tissue. 1,HSP70 Monoclonal Antibody（3G10）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-lung tissue. 1,HSP70 Monoclonal Antibody（3G10）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-testis tissue. 1,HSP70 Monoclonal Antibody（3G10）（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1） Hela, 2） Mouse Brain, diluted at 1:2000.

IF analysis of Hela with antibody （Left） and DAPI （Right） diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using Mouse mAb diluted at 1:500.

Western blot analysis of Pig Skeletal Muscle with HSP70 mAb diluted at 1:2,000.

Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4° over night, secondary antibody was diluted at 1:10000, 37° 1hour.