BD-PM3041-Bcl-2(6B5)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Bcl-2(6B5)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PM3041

产品名称

Bcl-2(6B5)Mouse Monoclonal Antibody

别名

BCL2; Apoptosis regulator Bcl-2

类别

常规抗体

基因名称

BCL2

蛋白名称

Apoptosis regulator Bcl-2

推荐应用

反应种属

Human,Mouse,Rat,chicken

浓度

0.73mg/mL

存储缓冲液

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

Human Gene ID

596

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=596

Human Swissprot No.

P10415

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P10415/entry

Mouse Gene ID

12043

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=12043

Mouse Swissprot No.

P10417

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P10417

Rat Gene ID

24224

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24224

Rat Swissprot No.

P49950

Rat Swissprot Link

http://www.uniprot.org/uniprot/P49950

免疫原

Synthetic Peptide of human Bcl-2 AA range: 1-100

特异性

The antibody detects endogenous Bcl-2 proteins.

稀释度

IF 1:50-200 WB 1:1000~2000 IHC 1:200

预测分子量

26kD

运输及保存条件

-20°C/1 year

宿主

Monoclonal, Mouse

背景介绍

BCL2, apoptosis regulator(BCL2) Homo sapiens This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],

组织表达

Expressed in a variety of tissues.

细胞定位

Mitochondrion outer membrane ; Single-pass membrane protein . Nucleus membrane ; Single-pass membrane protein . Endoplasmic reticulum membrane ; Single-pass membrane protein . Cytoplasm .

信号通路

Apoptosis_Inhibition;Apoptosis_Mitochondrial;Apoptosis_Overview;Focal adhesion;Neurotrophin;Amyotrophic lateral sclerosis (ALS);Pathways in cancer;Colorectal cancer;Prostate cancer;Small cell lung cancer;

功能

disease:A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.,domain:The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.,function:Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).,online information:Bcl-2 entry,PTM:Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A).,PTM:Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.,similarity:Belongs to the Bcl-2 family.,subunit:Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.,tissue specificity:Expressed in a variety of tissues.,

期货

现货

纯化

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Western blot analysis of Hela, diluted at 1:1000

IHC staining of Human tonsil tissue paraffin-embedded, diluted at 1:200.

The picture was kindly provided by our customer

Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000

Western blot detection of Bcl-2 in human breast cancer cell line MCF-7（A）, MDA-MB-231（B） and Cal51（C） using Bcl-2 mouse mAb （1:2000 diluted）.Predicted band size: 26kDa.Observed band size:26kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital

Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1, Using Bcl-2 Mouse mAb diluted at 1:200 （4°,overnight）. 2, High-pressure and temperature Citric acid, pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 50min）. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital

Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1, Antibody was diluted at 1:400（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.