产品名称
Topo IIIβ-1 Rabbit Polyclonal Antibody
别名
TOP3B; TOP3B1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1
蛋白名称
DNA topoisomerase 3-beta-1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=8940
Human Swissprot No.
O95985
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O95985/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=21976
Mouse Swissprot No.
Q9Z321
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9Z321
免疫原
The antiserum was produced against synthesized peptide derived from human TOP3B. AA range:51-100
特异性
Topo IIIβ-1 Polyclonal Antibody detects endogenous levels of Topo IIIβ-1 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013],
组织表达
Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.
细胞定位
condensed chromosome,nucleus,cytosol,
信号通路
Homologous recombination;
功能
catalytic activity:ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.,function:Possesses negatively supercoiled DNA relaxing activity.,similarity:Belongs to the prokaryotic type I/III topoisomerase family.,tissue specificity:Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
