BD-PT4659-TIMP-2 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

TIMP-2 Rabbit Polyclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PT4659

产品名称

TIMP-2 Rabbit Polyclonal Antibody

别名

TIMP2; Metalloproteinase inhibitor 2; CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2

类别

常规抗体

基因名称

TIMP2

蛋白名称

Metalloproteinase inhibitor 2

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

7077

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7077

Human Swissprot No.

P16035

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P16035/entry

Mouse Swissprot No.

P25785

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P25785

Rat Gene ID

29543

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29543

Rat Swissprot No.

P30121

Rat Swissprot Link

http://www.uniprot.org/uniprot/P30121

免疫原

The antiserum was produced against synthesized peptide derived from human TIMP2. AA range:21-70

特异性

TIMP-2 Polyclonal Antibody detects endogenous levels of TIMP-2 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

预测分子量

25kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008],

组织表达

Placenta,Skin,Synovial fluid,Testis,

细胞定位

Secreted.

功能

function:Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.,PTM:The activity of TIMP2 is dependent on the presence of disulfide bonds.,similarity:Belongs to the protease inhibitor I35 (TIMP) family.,similarity:Contains 1 NTR domain.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using TIMP2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, using TIMP2 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using TIMP-2 Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.