BD-PT4648-Thymidine Kinase Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Thymidine Kinase Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4648

产品名称

Thymidine Kinase Rabbit Polyclonal Antibody

别名

TK1; Thymidine kinase; cytosolic

类别

常规抗体

基因名称

TK1

蛋白名称

Thymidine kinase cytosolic

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

7083

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7083

Human Swissprot No.

P04183

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P04183/entry

Mouse Gene ID

21877

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=21877

Mouse Swissprot No.

P04184

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P04184

Rat Gene ID

24834

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=24834

Rat Swissprot No.

P27158

Rat Swissprot Link

http://www.uniprot.org/uniprot/P27158

免疫原

The antiserum was produced against synthesized peptide derived from human TK. AA range:1-50

特异性

Thymidine Kinase Polyclonal Antibody detects endogenous levels of Thymidine Kinase protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

25kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

catalytic activity:ATP + thymidine = ADP + thymidine 5'-phosphate.,miscellaneous:Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.,PTM:Phosphorylated on Ser-13 in mitosis.,similarity:Belongs to the thymidine kinase family.,

组织表达

Epithelium,Lymph,Skin,Uterus,

细胞定位

Cytoplasm.

信号通路

Pyrimidine metabolism;Drug metabolism;

功能

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of HepG2 cells, using TK Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TK Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from COLO205 cells, using TK Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.