产品名称
TFIIIA Rabbit Polyclonal Antibody
别名
GTF3A; Transcription factor IIIA; TFIIIA
蛋白名称
Transcription factor IIIA
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=2971
Human Swissprot No.
Q92664
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q92664/entry
Mouse Swissprot No.
Q8VHT7
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8VHT7
免疫原
Synthesized peptide derived from the Internal region of human TFIIIA.
特异性
TFIIIA Polyclonal Antibody detects endogenous levels of TFIIIA protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. [provided by RefSeq, Jul 2008],
功能
caution:It is uncertain whether Met-1 is the initiator. Based on the lack of an in-frame AUG codon, mammalian TFIIIA may be translated from this non-AUG initiation site, which has a good Kozak context and which is well conserved among mammals.,function:Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes.,sequence caution:Translation N-terminally shortened.,similarity:Contains 9 C2H2-type zinc fingers.,tissue specificity:Ubiquitous.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
