产品名称
TFE3 Rabbit Polyclonal Antibody
别名
TFE3; BHLHE33; Transcription factor E3; Class E basic helix-loop-helix protein 33; bHLHe33
蛋白名称
Transcription factor E3
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7030
Human Swissprot No.
P19532
Human Swissprot Link
http://www.uniprot.org/uniprotkb/P19532/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=209446
Mouse Swissprot No.
Q64092
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q64092
免疫原
The antiserum was produced against synthesized peptide derived from human TFE3. AA range:101-150
特异性
TFE3 Polyclonal Antibody detects endogenous levels of TFE3 protein.
稀释度
WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013],
组织表达
Ubiquitous in fetal and adult tissues.
细胞定位
Cytoplasm, cytosol . Nucleus . When nutrients are present, phosphorylation by MTOR prevents nuclear translocation and activity (PubMed:22692423, PubMed:30733432). Conversely, inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus (PubMed:22692423, PubMed:30733432). .
功能
disease:Chromosomal aberrations involving TFE3 are recurrent in alveolar soft part sarcoma (ASPS) [MIM:606243]. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.,disease:Chromosomal aberrations involving TFE3 are recurrent in alveolar soft part sarcoma (ASPS). Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.,disease:Chromosomal aberrations involving TFE3 may be a cause of papillary renal cell carcinoma (PRCC) [MIM:605074]. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.,function:Positive-acting transcription factor that binds to the immunoglobulin enchancer MUE3 motif. It also binds very well to a USF/MLTF site. Binding of TFE3 to DNA induces DNA binding.,similarity:Belongs to the MiT/TFE family.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,tissue specificity:Ubiquitous in fetal and adult tissues.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
