产品名称
TESK1 Rabbit Polyclonal Antibody
别名
TESK1; Dual specificity testis-specific protein kinase 1; Testicular protein kinase 1
蛋白名称
Dual specificity testis-specific protein kinase 1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=7016
Human Swissprot No.
Q15569
Human Swissprot Link
http://www.uniprot.org/uniprotkb/Q15569/entry
Mouse Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=21754
Mouse Swissprot No.
O70146
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O70146
Rat Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=29460
Rat Swissprot Link
http://www.uniprot.org/uniprot/Q63572
免疫原
The antiserum was produced against synthesized peptide derived from human TESK1. AA range:181-230
特异性
TESK1 Polyclonal Antibody detects endogenous levels of TESK1 protein.
稀释度
WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
testis-specific kinase 1(TESK1) Homo sapiens This gene product is a serine/threonine protein kinase that contains an N-terminal protein kinase domain and a C-terminal proline-rich domain. Its protein kinase domain is most closely related to those of the LIM motif-containing protein kinases (LIMKs). The encoded protein can phosphorylate myelin basic protein and histone in vitro. The testicular germ cell-specific expression and developmental pattern of expression of the mouse gene suggests that this gene plays an important role at and after the meiotic phase of spermatogenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015],
组织表达
Expressed in podocytes and renal tubular cells in the kidney (at protein level).
细胞定位
Cytoplasm . Cytoplasm, perinuclear region . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cell projection, lamellipodium . Colocalizes with SPRY4 in vesicular spots in the cytoplasm (PubMed:15584898). Localized to F-actin-rich lamellipodia at the cell periphery following fibronectin-mediated cell adhesion of Schwann cells (By similarity). .
信号通路
Regulation of Microtubule Dynamics
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,cofactor:Manganese.,domain:The extracatalytic C-terminal part is highly rich in proline residues.,enzyme regulation:Activated by autophosphorylation on Ser-220.,function:Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Probably plays a central role at and after the meiotic phase of spermatogenesis.,PTM:Autophosphorylated on serine and tyrosine residues.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with SPRY4.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
