BD-PT4594-TdT Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

TdT Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4594

产品名称

TdT Rabbit Polyclonal Antibody

别名

DNTT; TDT; DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase

类别

常规抗体

基因名称

DNTT

蛋白名称

DNA nucleotidylexotransferase

推荐应用

反应种属

Human,Rat,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1791

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1791

Human Swissprot No.

P04053

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P04053/entry

Mouse Swissprot No.

P09838

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P09838

免疫原

The antiserum was produced against synthesized peptide derived from human DNTT. AA range:381-430

特异性

TdT Polyclonal Antibody detects endogenous levels of TdT protein.

稀释度

IHC-p: 100-300.WB 1:500 - 1:2000. ELISA: 1:40000.. IF 1:50-200

预测分子量

60kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008],

组织表达

Testis,Thymus,

细胞定位

Nucleus .

信号通路

Non-homologous end-joining;Hematopoietic cell lineage;

功能

catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Magnesium.,disease:Very high levels of enzyme activity have been detected in certain acute leukemic cells.,function:Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.,similarity:Belongs to the DNA polymerase type-X family.,similarity:Contains 1 BRCT domain.,subunit:Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of DNTT Antibody. The lane on the right is blocked with the DNTT peptide.

Western Blot analysis of HepG2 cells using TdT Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human Thyroid. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.

Immunohistochemical analysis of paraffin-embedded Human thymus. 1, Antibody was diluted at 1:200（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.