BD-PT4522-TACC3 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

TACC3 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4522

产品名称

TACC3 Rabbit Polyclonal Antibody

别名

TACC3; ERIC1; Transforming acidic coiled-coil-containing protein 3; ERIC-1

类别

常规抗体

基因名称

TACC3

蛋白名称

Transforming acidic coiled-coil-containing protein 3

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

10460

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10460

Human Swissprot No.

Q9Y6A5

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q9Y6A5/entry

Mouse Swissprot No.

Q9JJ11

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q9JJ11

免疫原

The antiserum was produced against synthesized peptide derived from human TACC3. AA range:789-838

特异性

TACC3 Polyclonal Antibody detects endogenous levels of TACC3 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

80kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

This gene encodes a member of the transforming acidic colied-coil protein family. The encoded protein is a motor spindle protein that may play a role in stabilization of the mitotic spindle. This protein may also play a role in growth a differentiation of certain cancer cells. [provided by RefSeq, Nov 2011],

组织表达

Epithelium,PCR rescued clones,Skin,

细胞定位

Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . In complex with CKAP5 localized to microtubule plus-ends in mitosis and interphase. In complex with CKAP5 and clathrin localized to inter-microtubule bridges in mitotic spindles. .

功能

function:Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). May be involved in the control of cell growth and differentiation. May contribute to cancer.,induction:Up-regulated in various cancer cell lines.,similarity:Belongs to the TACC family.,subunit:Interacts with microtubules. Interacts with CCDC100/CEP120. The coiled coil C-terminus region interacts with AH receptor nuclear translocator protein (ARNT) and ARNT2 (By similarity). Interacts with GCN5L2 and PCAF.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using TACC3 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using TACC3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 and HUVEC cells, using TACC3 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using TACC3 Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100（4°,overnight）. 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200（room temperature, 30min）.