产品名称
E2F-3 (Acetyl-Lys168) Rabbit Polyclonal Antibody
蛋白名称
E2F transcription factor 3
反应种属
Human:K168,Mouse:K160,Rat:K161
存储缓冲液
PBS, pH 7.4, containing 0.02% New type preservative N as Preservative and 50% Glycerol.
Human Gene Link
http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1871
Human Swissprot No.
O00716
Human Swissprot Link
http://www.uniprot.org/uniprotkb/O00716/entry
Mouse Swissprot No.
O35261
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O35261
免疫原
Synthesized acetyl-peptide from human protein at AA range: 130-200
特异性
This antibody detects endogenous levels of E2F-3 at Human:K168;Mouse:K160;Rat:K161, It doesn't reacte with total protein.
稀释度
WB 1:500-10000 ELISA: 1:10000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013],
组织表达
Bone marrow,PCR rescued clones,Placenta,Pre-B cell,Rectum tumor,Uterus,
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Pathways in cancer;Pancreatic cancer;Glioma;Prostate cancer;Melanoma;Bladder cancer;Chronic myeloid leukemia;Small cell lung cancer;Non-small cell lung cancer;
功能
function:Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-3 binds specifically to RB1 protein, in a cell-cycle dependent manner.,similarity:Belongs to the E2F/DP family.,subunit:Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
