BD-PT4417-SRp20 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SRp20 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4417

产品名称

SRp20 Rabbit Polyclonal Antibody

别名

SRSF3; SFRS3; SRP20; Serine/arginine-rich splicing factor 3; Pre-mRNA-splicing factor SRP20; Splicing factor; arginine/serine-rich 3

类别

常规抗体

基因名称

SRSF3

蛋白名称

Serine/arginine-rich splicing factor 3

推荐应用

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

6428

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6428

Human Swissprot No.

P84103

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P84103/entry

Mouse Gene ID

20383

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20383

Mouse Swissprot No.

P84104

Mouse Swissprot Link

http://www.uniprot.org/uniprot/P84104

免疫原

The antiserum was produced against synthesized peptide derived from human SFRS3. AA range:111-160

特异性

SRp20 Polyclonal Antibody detects endogenous levels of SRp20 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

25kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. [provided by RefSeq, Sep 2010],

组织表达

Brain,Epithelium,Placenta,Tongue,

细胞定位

Nucleus . Nucleus speckle . Cytoplasm . Recruited to nuclear speckles following interaction with YTHDC1. .

信号通路

Spliceosome;

功能

function:May be involved in RNA processing in relation with cellular proliferation and/or maturation.,PTM:Extensively phosphorylated on serine residues in the RS domain.,similarity:Belongs to the splicing factor SR family.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with CPSF6, RBMY1A1 and SFRS12.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of MCF7 cells, using SFRS3 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa and Jurkat cells, using SFRS3 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using SRp20 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of Jurkat cells using SRp20 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western blot analysis of KB lysis using SRp20 antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western blot analysis of 293T lysis using SRp20 antibody. Antibody was diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.