BD-PT4385-Spindlin-1 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Spindlin-1 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4385

产品名称

Spindlin-1 Rabbit Polyclonal Antibody

别名

SPIN1; OCR; SPIN; Spindlin-1; Ovarian cancer-related protein

类别

常规抗体

基因名称

SPIN1

蛋白名称

Spindlin-1

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

10927

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=10927

Human Swissprot No.

Q9Y657

Human Swissprot Link

http://www.uniprot.org/uniprotkb/Q9Y657/entry

Mouse Gene ID

20729

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20729

Mouse Swissprot No.

Q61142

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q61142

Rat Gene ID

100364000

Rat Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=100363675

Rat Swissprot No.

Q4V8J7

Rat Swissprot Link

http://www.uniprot.org/uniprot/Q4V8J7

免疫原

The antiserum was produced against synthesized peptide derived from human SPIN1. AA range:111-160

特异性

Spindlin-1 Polyclonal Antibody detects endogenous levels of Spindlin-1 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

预测分子量

30kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

function:May play a role in cell-cycle regulation during the transition from gamete to embryo.,PTM:Phosphorylated during oocyte meiotic maturation.,sequence caution:Translated as Glu.,similarity:Belongs to the SPIN/STSY family.,

组织表达

Highly expressed in ovarian cancer tissues.

细胞定位

Nucleus . Nucleus, nucleolus .

功能

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Western blot analysis of lysates from HeLa cells, using SPIN1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from K562 cells using SPIN1 antibody.

Western Blot analysis of various cells using Spindlin-1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Western Blot analysis of HeLa cells using Spindlin-1 Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100（4°,overnight）. High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl （right） obtaned from antibody was pre-absorbed by immunogen peptide.