BD-PT4371-Sox-9 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Sox-9 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PT4371

产品名称

Sox-9 Rabbit Polyclonal Antibody

别名

SOX9; Transcription factor SOX-9

类别

常规抗体

基因名称

SOX9

蛋白名称

Transcription factor SOX-9

推荐应用

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

6662

Human Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=6662

Human Swissprot No.

P48436

Human Swissprot Link

http://www.uniprot.org/uniprotkb/P48436/entry

Mouse Gene ID

20682

Mouse Gene Link

http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=20682

Mouse Swissprot No.

Q04887

Mouse Swissprot Link

http://www.uniprot.org/uniprot/Q04887

免疫原

The antiserum was produced against synthesized peptide derived from human SOX9. AA range:147-196

特异性

Sox-9 Polyclonal Antibody detects endogenous levels of Sox-9 protein.

稀释度

WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

预测分子量

65kD

运输及保存条件

-20°C/1 year

宿主

Polyclonal, Rabbit,IgG

背景介绍

SRY-box 9(SOX9) Homo sapiens The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008],

组织表达

Eye,PNS,Testis,

细胞定位

Nucleus .

功能

disease:Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.,function:Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.,similarity:Contains 1 HMG box DNA-binding domain.,

期货

现货

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunofluorescence analysis of A549 cells, using SOX9 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SOX9 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with PBS 60‘, using SOX9 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of rat-lung tissue. 1,Sox-9 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-spleen tissue. 1,Sox-9 Polyclonal Antibody（red） was diluted at 1:200（4°C,overnight）. 2, Cy3 labled Secondary antibody was diluted at 1:300（room temperature, 50min）.3, Picture B: DAPI（blue） 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western Blot analysis of various cells using Sox-9 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit （SC-003,Inventbiotech,MN,USA）.